Conventional and liquid-based cytology (Liqui-PREP™) anal findings in male HIV anoreceptive patients in a referral University hospital in Rio de Janeiro, Brazil

Authors

  • Simone Maia Evaristo Instituto Nacional de Câncer José Alencar Gomes da Silva
  • Gysele Guimarães Carvalho Rocha Instituto Nacional de Câncer José Alencar Gomes da Silva
  • Laís Emanauella da Silva Alves Instituto Nacional de Câncer José Alencar Gomes da Silva
  • Neimar de Paula Silva Instituto Nacional de Câncer José Alencar Gomes da Silva
  • Mario Lucio Cordeiro Araújo Junior Instituto Nacional de Câncer José Alencar Gomes da Silva
  • Jorge Francisco da Cunha Pinto Universidade Federal do Estado do Rio de Janeiro
  • Cassia Cristina Alves Gonçalves Universidade Federal do Estado do Rio de Janeiro
  • Rodrigo Siqueira da Rocha Dias Universidade Federal Fluminense - UFF
  • José Eleutério Junior Universidade Federal do Ceará

Keywords:

anal neoplasm, cytological techniques, HIV, male homosexuality

Abstract

Introduction: The anal lesions seem to have a natural history that closely resembles cervical lesions, with signs that precede the invasion. Cytological changes of anal epithelium induced by HPV can be detected through cytology, as it is considered an effective screening method. Objective: To identify the frequency of atypical epithelial conventional cytology results by comparing anal samples through Liqui-PREPTM technology in HIV-positive men. Methods: Cross-sectional descriptive and analytical study of 33 men who have sex with men (MSM), HIV-positive and anoreceptive attended at the Gaffrèe and Guinle University Hospital (HUGG), Rio de Janeiro, from June to July, 2016. Collection of anal samples for the conventional cytology and Liqui-PREPTM cytology was carried out. For significance of findings, Fisher exact test with 95% confidence interval was used and cytological Kappa index was employed for concordance between the two cytological methods. Results: The age ranged from 23 to 60 years (mean=39.06). The CD4 cell count was between 200 to 500/mm3 on 16 (48.5%) and 13 (39.4%), and 50% was diagnosed with HIV for more than 6 years. In conventional cytology one case was considered unsatisfactory (3%). Among the cases considered satisfactory, 9 (28.1%) were diagnosed with ASC-US; 4 (12.5%) LSIL; 2 (6.3%) ASC-H, and 2 (6.3%) HSIL. Through Liqui-PREPTM method, 7 cases were considered unsatisfactory (21.2%). Among the satisfactory cases, 7 showed ASC-US (26.9%); 4 (15.4%) ASC-H; 2 (7.7%) LSIL; and 2 (7.7%) HSIL. The difference of unsatisfactory cases between both methods, although higher for Liqui-PREPTM, was not statistically significant (p=0.054). The correlation was moderate (0503; p<0.006 [0.1765–0.8298]). Conclusion: The cytologic atypia is common among MSM HIV (+), and the anal conventional cytology and liquid by Liqui-PREPTM cytology are equivalent, although they are more unsatisfactory in the latter technique.

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Author Biographies

Simone Maia Evaristo, Instituto Nacional de Câncer José Alencar Gomes da Silva

Instituto Nacional de Câncer José Alencar Gomes da Silva – Rio de Janeiro (RJ), Brazil.

Gysele Guimarães Carvalho Rocha, Instituto Nacional de Câncer José Alencar Gomes da Silva

Instituto Nacional de Câncer José Alencar Gomes da Silva – Rio de Janeiro (RJ), Brazil.

Laís Emanauella da Silva Alves, Instituto Nacional de Câncer José Alencar Gomes da Silva

Instituto Nacional de Câncer José Alencar Gomes da Silva – Rio de Janeiro (RJ), Brazil.

Neimar de Paula Silva, Instituto Nacional de Câncer José Alencar Gomes da Silva

Instituto Nacional de Câncer José Alencar Gomes da Silva – Rio de Janeiro (RJ), Brazil.

Mario Lucio Cordeiro Araújo Junior, Instituto Nacional de Câncer José Alencar Gomes da Silva

Instituto Nacional de Câncer José Alencar Gomes da Silva – Rio de Janeiro (RJ), Brazil.

Jorge Francisco da Cunha Pinto, Universidade Federal do Estado do Rio de Janeiro

Centro de Ciências Biológicas e da Saúde, Universidade Federal do Estado do Rio de Janeiro – Rio de Janeiro (RJ), Brazil.

Cassia Cristina Alves Gonçalves, Universidade Federal do Estado do Rio de Janeiro

Centro de Ciências Biológicas e da Saúde, Universidade Federal do Estado do Rio de Janeiro – Rio de Janeiro (RJ), Brazil.

Rodrigo Siqueira da Rocha Dias, Universidade Federal Fluminense - UFF

Universidade Federal Fluminense – Niterói (RJ), Brazil

José Eleutério Junior, Universidade Federal do Ceará

Departamento de Saúde Materno Infantil, Universidade Federal do Ceará – Fortaleza (CE), Brazil

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Published

2018-03-10

How to Cite

1.
Evaristo SM, Rocha GGC, Alves LE da S, Silva N de P, Araújo Junior MLC, Pinto JF da C, et al. Conventional and liquid-based cytology (Liqui-PREP™) anal findings in male HIV anoreceptive patients in a referral University hospital in Rio de Janeiro, Brazil. DST [Internet]. 2018 Mar. 10 [cited 2024 Jul. 12];30(1):20-4. Available from: https://www.bjstd.org/revista/article/view/814

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Original Article