Frequency and genotyping of human papillomavirus in women submitted to citology

Authors

  • Emmanuele Pariz Silva
  • Giovanna Grünewald Vietta
  • Lisléia Golfetto
  • Marco Antonio Zonta
  • Eloisa Regina Gularte
  • Maria Elisabeth Menezes
  • Daiane Cobianchi

Keywords:

papillomaviridae, polymerase chain reaction, sexually transmitted diseases

Abstract

Introduction: Among the sexually transmitted virus, the human papilloma virus (HPV) is the most prevalent and may be detected a considerable number of sexually active women. He is considered the main agent of cervical cancer. Therefore, the high-risk HPV identification can aid in the prevention of cervical lesions. Objective: To evaluate the occurrence of HPV infections, comparing different methodologies, as well as some risk factors and their potential association in the development of cervical cancer in women submitted to cytopathology treated in ambulatory Unit Family and Community Health (USFC) of the University of Vale do Itajaí (UNIVALI). Methods: 118 samples were evaluated sexually active women who sought care for screening of cervical cancer in USFC and UNIVALI. All samples were subjected to polymerase chain reaction (PCR) and the liquid and conventional cytology. However, only 64 women were subjected to hybrid capture methodology (CH2). Results: The prevalence of HPV was 43.22% by PCR and 25% for CH2; analysis of the results was observed association between HPV and the following variables: ethnicity (p<0.016), scholarity (p<0.012), human immunodeficiency virus (HIV) (p<0.008), preservative (p<0.02), oral contraceptives (p<0.03), younger age at first sexual intercourse (p<0.07), conventional cytology (p<0.002) and liquid cytology (p<0.029). Conclusion: The incidence of HPV infection is high and the high-risk HPV was primarily associated with the younger age at first sexual intercourse.

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Author Biographies

Emmanuele Pariz Silva

Undergraduate Student in the Biomedicine Course, Universidade do Vale do Itajaí (UNIVALI) – Itajaí (SC), Brazil.

Giovanna Grünewald Vietta

Professor in the Biomedicine Course, UNIVALI – Itajaí (SC), Brazil

Lisléia Golfetto

Graduate Student in the Graduate Program in Pharmacy, Universidade Federal de Santa Catarina (UFSC) – Florianópolis (SC), Brazil.

Marco Antonio Zonta

PhD in Infectious Diseases, Universidade Federal de São Paulo (UNIFESP). Director of Laboratório IN CITO – São Paulo (SP), Brazil.

Eloisa Regina Gularte

Professor in the Biomedicine Course, UNIVALI – Itajaí (SC), Brazil.

Maria Elisabeth Menezes

President of Instituto de Biologia Molecular Aplicada (IBIOTECNO). Director of Laboratório de Análise e Pesquisa do Gene (DNAnálise) – Florianópolis (SC), Brazil.

Daiane Cobianchi

Professor in the Biomedicine Course, UNIVALI – Itajaí (SC), Brazil

Published

2022-04-20

How to Cite

1.
Silva EP, Vietta GG, Golfetto L, Zonta MA, Gularte ER, Menezes ME, et al. Frequency and genotyping of human papillomavirus in women submitted to citology. DST [Internet]. 2022 Apr. 20 [cited 2024 Mar. 29];27(1-2):22-8. Available from: https://www.bjstd.org/revista/article/view/692

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Section

Original Article